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Image Search Results
Journal: Cells
Article Title: Maternal Exercise Mediates Hepatic Metabolic Programming via Activation of AMPK-PGC1α Axis in the Offspring of Obese Mothers
doi: 10.3390/cells10051247
Figure Lengend Snippet: Maternal exercise before and during pregnancy alters the body composition of obese dams. ( A ) Experimental design: WSD, sedentary obese dams; WSD-Run, obese dams performing voluntary wheel running before and during pregnancy. ( B ) Induction of a model of maternal obesity. Female mice (n = 86) were fed an obesogenic western style diet for 8 weeks for induction of an obese phenotype and a cohort of obese dams, respectively. A separate cohort (n = 62) of female mice receiving a standard laboratory chow diet served as lean control group (Control). ( C ) Glucose tolerance was measured via intraperitoneal glucose tolerance test (ipGTT) at week 8–9 indicating altered glucose tolerance in obese dams ( n = 10/group). Thus, before subsequent division into an ‘exercise’ and ‘sedentary’ group, the maternal obesity cohort exhibited a disturbed metabolic phenotype with WSD induced obesity and impaired glucose metabolism. ( D ) Weight gain during pregnancy. ( E ) Average running distance of the exercise group (WSD-Run) before and during pregnancy in km/day. ( F ) Total body weight of dams at the end of lactation at postnatal day (P) 21 (WSD group (n = 24), WSD-Run group (n = 22)). ( G ) Epigonadal fat pad weight at P21 (WSD group (n = 22), WSD-Run group (n = 20)). Data are presented as mean ± standard deviation; * p < 0.05, ** p < 0.01, **** p < 0.0001. BW, body weight; egWAT, epigonadal white adipose tissue; p, postnatal day; WSD, western-style diet; WSD-Run, western-style diet induced obese dams performing voluntary wheel running.
Article Snippet: First, three-week-old female C57BL/6N mice, obtained from Charles River Laboratories (Germany), were fed an energy-rich
Techniques: Western Blot, Control, Standard Deviation